It has been well known that lncRNAs are important players in the regulation of several aspects of cellular biology, and determining their tissue or cell specificity is fundamental to better comprehension of their function. To better explore the spatial expression patterns of lncRNAs at single-cell level across normal and cancer tissus, we have updated our already rich LncSpA (covering 21,230 samples across 38 normal tissues and 40 cancer types, and 12,007 TE lncRNAs and 634 clinical-related TE lncRNAs were also included), raising its collection to about 1.1 millions of single cells. LncSpA V2.0 is based on 1,166,965 single cell samples, covering 185 cell types across 23 cancer types and 41 normal tissues of the human body, and the currently published scRNA-seq data by the project of human cell landscape were also collected. Similarly as the method to identify tissue-elevated (TE) lncRNAs in our previous study, the cell-elevated (CE) lncRNAs were comprehensively discovered, which were further classified into cell specific(CS), cell enriched(CER) and cell enhanced(CEH). Currently，17,600 CE lncRNAs were included in LncSpA V2.0.
1.2 Definition of lncNRA classification
2.1 Cluster of single cells
(1) Click on tissue or cancer and enter the gene.
You can browse the clustering of single cells by clicking on different tissues or cancers and the corresponding resources, and you can browse the expression of a gene in a single cell by typing in the gene of interest.
2.2 Four tree centric
You can browse the right side of the tree centric for the distribution of all lncRNA in normal tissue and cancer from various sources.
(1) lncRNA centric.
(2) normal centric.
(3) cancer centric.
(4) clinical centric.
Four ways were provided to scan the information of your interested lncRNA or lncRNA set in detail.
3.1 Normal tissue.
In this module, you can select single cell or bulk transcriptome from normal tissues, and you can can select a resource, tissue and category of interest to view the informations of lncRNAs.
In this module, you can select single cell or bulk transcriptome from cancer, and you can can select a resource, tissue and category of interest to view the informations of lncRNAs.
In this module, you can select single cell or bulk transcriptome, and you can input lncRNA ensembl ID or symbol to view the informations of lncRNAs from all resource.
In this module, you can select single cell or bulk transcriptome, and you can input genomic position of interest to view the informations of lncRNAs.
4.Result and details
4.1 Table of results.
In this moudle, you can visit some basic information based on resource, tissue, classification and cell type queries.