Detail

Chr	chr22
start	27221349
end	27224727
lncRNA name	LINC01638
entrez id	105372978
hgnc id	HGNC:52425
ensg id	ENSG00000233521
refseq id	NA
methods	qRT-PCR, Western blot assay, ChIP, Luciferase reporter assay, RIP
regulated	up-regulated
function description	LINC01638 is highly expressed in TNBC tissues and cells.Mechanistically, LINC01638 interacts with c-Myc to prevent SPOP-mediated c-Myc ubiquitination and degradation. C-Myc transcriptionally enhances MTDH (metadherin) expression and subsequently activates Twist1 expression to induce EMT. Analysis of the epithelial marker E-cadherin and mesenchymal markers Vimentin and EMT-associated transcription factor Twist1 revealed that LINC01638 knockdown increased E-cadherin and reduced Vimentin and Twist1 expression at the mRNA (Fig. 2g) and protein (Fig. 2h) levels in MDA-MB-231 and BT549 cells, whereas LINC01638 overexpression showed an inverse effect in T47D cells (Fig. 2g, h).
pubmed id	30002443
year	2018
title	LINC01638 lncRNA activates MTDH-Twist1 signaling by preventing SPOP-mediated c-Myc degradation in triple-negative breast cancer.
drug	X
circulating	X
survival	V