Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieRe-Programming Of Cho Cells
CeRNA1miR-23 sponge[Artifically engineered RNA]
miRNAmiR-23[miRNA]
CeRNA2IDH1[mRNA]
Tissue/Cell lineCho Cells
SpecieMus musculus (mouse)
CitationBiotechnol J 2015 Jul 10, 1029-40 doi:10.1002/biot.201500101 PMID:26097147
Reference title
Re-programming CHO cell metabolism using miR-23 tips the balance towards a highly productive phenotype.
Experimental verification
luciferase reporter assays;qRT-PCR;Western blot assay
Functional description
CHO clones depleted of miR-23 using a miR-sponge decoy demonstrated an average ~three-fold enhanced specific productivity with no impact on cell growth. Using a cell respirometer, mitochondrial activity was found to be enhanced by ~30% at Complex I and II of the electron transport system. Additionally, label-free proteomic analysis uncovered various potential novel targets of miR-23 including LE1 and IDH1, both implicated in oxidative metabolism and mitochondrial activity.
Annotations
External Annotation for miR-23 sponge |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
