Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieBreast Cancer
CeRNA1miR-183 sponge[LncRNA]
miRNAmiR-183[miRNA]
CeRNA2RAB21[mRNA]
Tissue/Cell lineMcf-7,Mda-Mb-231,Sk-Br-3,T47D, Zr-75-1, Mcf-10A
SpecieHomo sapiens (human)
CitationBreast Cancer Res 2014 Nov 14 16, 473 doi:10.1186/s13058-014-0473-z PMID:25394902
Reference title
MiR-183/-96/-182 cluster is up-regulated in most breast cancers and increases cell proliferation and migration.
Experimental verification
luciferase reporter assays;qRT-PCR;Western blot assay
Functional description
To investigate the biological effects of the miR-183/-96 /-182 cluster in breast cancer, we generated miR-96, miR-182 and miR-183 overexpression stable cell lines to check the overdose effects; we also used miR-Down antagomir for each miRNA as well as miR-183/-96 /-182 cluster sponge lentivirus to check the knockdown effects.The data showed that RAB21 protein was significantly decreased in the miR-183 overexpression cell lines, but not in miR-96 and miR-182 overexpression cell lines compared with the empty vector control cell lines and wild-type cells
Annotations
External Annotation for miR-183 sponge |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
