Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieOsteoblast Differentiation
CeRNA1H19[LncRNA]
miRNAmiR-22[miRNA]
CeRNA2CTNNB1[mRNA]
Tissue/Cell lineHek293,Hmscs
SpecieHomo sapiens (human)
CitationSci Rep 2016 Feb 8 6, 20121 doi:10.1038/srep20121 PMID:26853553
Reference title
H19 activates Wnt signaling and promotes osteoblast differentiation by functioning as a competing endogenous RNA.
Experimental verification
luciferase reporter assays;qRT-PCR;Western blot assay
Functional description
Meanwhile, by using bioinformatic investigations and RIP assays combined with luciferase reporter assays, we demonstrated that H19 functioned as an miRNA sponge for miR-141 and miR-22, both of which were negative regulators of osteogenesis and Wnt/b-catenin pathway. Further investigations revealed that H19 antagonized the functions of these two miRNAs and led to de-repression of their shared target gene b-catenin, which eventually activated Wnt/b-catenin pathway and hence potentiated osteogenesis.
Annotations
External Annotation for H19 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
