Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieLaryngeal Squamous Cell Cancer
CeRNA1AC026166.2-001[LncRNA]
miRNAmiR-24-3p[miRNA]
CeRNA2p27[mRNA]
Tissue/Cell lineAmc-Hn-8, Tu-212, Xenograft Tumor Tissues
SpecieHomo sapiens (human)
CitationSci Rep 2018 Feb 20 8, 3375 doi:10.1038/s41598-018-21659-5 PMID:29463827
Reference title
Long non-coding RNA AC026166.2-001 inhibits cell proliferation and migration in laryngeal squamous cell carcinoma by regulating the miR-24-3p/p27 axis.
Experimental verification
Microarray,PCR,Western blot,Luciferase reporter assay,knockdown
Functional description
AC026166.2-001 inhibited LSCC cell proliferation and the clone-forming capacity.Cell cycle distribution and related protein changes were measured by flow cytometry.AC026166.2-001 arrested the cell cycle at the G1 phase and induced apoptosis.In addition, AC026166.2-001 decreased cell migration as measured by wound healing assays and transwell migration assays.Moreover,luciferase reporter assay and Western blotting results suggested that AC026166.2-001 acts as a sponge of miR-24-3p and regulates the expression of p27 and cyclin D1.The in vivo results showed that AC026166.2-001 significantly suppressed the growth of LSCC xenografts and promoted apoptosis.overexpression of miR-24-3p significantly reduced the luciferase activity of AC026166.2-001 WT GP-miRGLO vectors but not of AC026166.2-001 MUT.Next,AC026166.2-001 may act as a sponge for miR-24-3p.Quantitative RT-PCR was used to test our assumption and we found that miR-24-3p was down-regulated by AC026166.2-001.
Annotations
External Annotation for AC026166.2-001 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
