Basic Information
Regular Relationship :
Tissue/Cell lineMg-63 And Os-732,Hfob1.19
SpecieHomo sapiens (human)
CitationCell Physiol Biochem 2018 45, 844-855 doi:10.1159/000487178 PMID:29414815
Reference title
LncRNA GAS5 Represses Osteosarcoma Cells Growth and Metastasis via Sponging MiR-203a.
Experimental verification
qPCR,Western blot
Functional description
LncRNA GAS5 was down-regulated in MG-63 and OS-732 cells compared to hFOB1.19 cells.Silence of lncRNA GAS5 significantly promoted MG-63 cells viability,migration and invasion,and up-regulated Cyclin D1,Cyclin B1,CDK1 and CDK4 expressions.miR-203a was negatively regulated by lncRNA GAS5.The promoting activities of lncRNA GAS5 silence on MG-63 cells growth and metastasis were reversed by miR-203a suppression.lncRNA GAS5 silence,miR-203a overexpression,and TIMP2 silence could activate PI3K/AKT/GSK3β signaling while block NF-κB signaling.LncRNA GAS5 might be a tumor suppressor in osteosarcoma via sponging miR-203a,sequestering miR-203a away from TIMP2.
Annotations
External Annotation for GAS5 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
