Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieEsophageal Cancer
CeRNA1GAS5[LncRNA]
miRNAmiR-301a[miRNA]
CeRNA2CXCR4[mRNA]
Tissue/Cell lineEca109, Te-1, Te-3 And Ec9706, Esophageal Epithelia Cell Line
SpecieHomo sapiens (human)
CitationOncol Res 2018 Sep 14 26, 1285-1294 doi:10.3727/096504018x15166193231711 PMID:29386089
Reference title
Long non-coding RNA GAS5 promotes proliferation,migration and invasion by regulation of miR-301a in esophageal cancer.
Experimental verification
qRT-PCR,Western blot,Luciferase reporter assay,in vitro knockdown
Functional description
GAS5 expression was increased in EC cells (ECA109, TE-1, TE3 and EC9706) compared to SHEE cells. Knockdown of GAS5 decreased cell viability,migration,invasion,and induced apoptosis in EC9706 cells. Moreover, miR-301a was directly sponged to GAS5,and miR-301a suppression obviously alleviated the pro-tumor effects of GAS5.Furthermore,miR-301a positively regulated CXCR4 expression,and overexpression of CXCR4 induced apoptosis and abolished the promoting effect of miR-301a inhibition on cell viability,migration and invasion.Besides,miR-301a blocked Wnt/β-catenin and NF-κB signal pathways by regulation of CXCR4.
Annotations
External Annotation for GAS5 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
