Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieNon-Small Cell Lung Cancer
CeRNA1H19[LncRNA]
miRNAmiR-17[miRNA]
CeRNA2STAT3[mRNA]
Tissue/Cell lineA549, H1299 And Bes-2B
SpecieHomo sapiens (human)
CitationJ Cell Physiol 2018 Oct 233, 6768-6776 doi:10.1002/jcp.26530 PMID:29693721
Reference title
H19 promotes non-small-cell lung cancer (NSCLC) development through STAT3 signaling via sponging miR-17.
Experimental verification
qPCR,Western blot,Luciferase reporter assay etc
Functional description
H19 was upregulated in A549 and H1299 cells compared to normal lung epithelial BEAS-2B cells.Meanwhile,miR-17 was downregulated in NSCLC cell lines.Inhibited H19 can suppress the growth,migration,and invasion of NSCLC cells and bioinformatics search was performed to predict the correlation between H19 and miR-17.Overexpression of miR-17 was able to inhibit the progression of NSCLC cells while reversely miR-17 inhibitors reversed this process.In addition, signal transducers and activators of transcription (STAT3),as an mRNA target of miR-17,was presented in our research.Moreover, we discovered that H19 demonstrated its biological functions via regulating miR-17 and STAT3 in vitro.Silencing H19 greatly increased STAT3 expression by sponging miR-19 in vitro.It was hypothesized that H19 may serve as a competing endogenous RNA (ceRNA) to modulate STAT3 by attaching miR-17 in lung cancer.
Annotations
External Annotation for H19 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
