Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieProstate Cancer
CeRNA1HOTAIR[LncRNA]
miRNAmiR-454-3p[miRNA]
CeRNA2EZH2[mRNA]
Tissue/Cell lineDu145,Pc3
SpecieHomo sapiens (human)
CitationBiochim Biophys Acta Gen Subj 2018 Mar 1862, 589-599 doi:10.1016/j.bbagen.2017.12.001 PMID:29221985
Reference title
HOTAIR-mediated reciprocal regulation of EZH2 and DNMT1 contribute to polyphyllin I-inhibited growth of castration-resistant prostate cancer cells in vitro and in vivo.
Experimental verification
qPCR,Western blot,in vitro knockdown etc
Functional description
Silenced HOTAIR reduced EZH2 and DNMT1 protein expressions.On the contrary, exogenously expressed HOTAIR resisted PPI-inhibited EZH2 and DNMT1 protein expressions, EZH2 promoter activity and cell growth. Moreover, excessive EZH2 antagonized PPI-suppressed DNMT1 protein expression or vice versa.The interactions among HOTAIR, DNMT1 and EZH2, and reciprocal regulation of DNMT1 and EZH2 contribute to the overall responses of PPI. HOTAIR induced DNA methylation of miR-454-3p via recruiting EZH2 and DNMT1 to the promoter regions of miR-454-3p, this largely reduced miR-454-3p expression,thereby reducing apoptosis and inducing autophagy in human chondrosarcoma cells
Annotations
External Annotation for HOTAIR |
|
|---|---|
 |
LncRNA-associated competing triplets and functions. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
 |
Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
 |
Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
 |
Information on all annotated and predicted human genes. |
 |
Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
 |
Genome browser for vertebrate genomes. |
 |
An annotated collection of all publicly available DNA sequences. |
 |
A wiki-based platform for community curation of human long non-coding RNAs. |
 |
An integrated knowledge database dedicated to non-coding RNAs. |
 |
An integrated database of human annotated lncRNA transcripts. |
 |
Comprehensive annotations of eukaryotic long non-coding RNAs. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
 |
The catalogue of somatic mutations in cancer. |
