Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieCervical Cancer
CeRNA1HOTAIR[LncRNA]
miRNAmiR-331-3p[miRNA]
CeRNA2NRP2[mRNA]
Tissue/Cell lineMri-H196 And Mri-H186
SpecieHomo sapiens (human)
CitationJ Cell Biochem 2018 Jun 119, 4397-4407 doi:10.1002/jcb.26503 PMID:29130509
Reference title
ARFHPV E7 oncogene, lncRNA HOTAIR, miR-331-3p and its target, NRP2, form a negative feedback loop to regulate the apoptosis in the tumorigenesis in HPV positive cervical cancer.
Experimental verification
qPCR,Western blot,RNAi etc
Functional description
NRP2 was identified as a virtual target gene of miR-331-3p with a binding site of miR-331-3p,and HOTAIR was directly sponged to miR-331-3p,miR-331-3p reduced luciferase activity of wild-type of NRP2 3'UTR and HOTAIR, but not those of mutant NRP2 3'UTR and HOTAIR.MiR-331-3p down-regulated NRP2 and E7 expression levels,and further promoted cell apoptosis,while inhibited cell proliferation.Cell transfected with HPV16 E7 displayed lower levels of HOTAIR,NRP2 and P53,a higher level of miR-331-3p,over-expression of E7 further repressed cell apoptosis,while improved cell proliferation compared with control. Normal HPV (+) group exhibited a higher miR-331-3p, and lower mRNA levels of HOTAIR and NRP2 than HPV (-) group.NRP2 protein was highly expressed in HPV (-) group compared to that in HPV (+) group. E7-HOTAIR-miR-331-3p-NRP2-E7 formed a regulatory loop, and could be involved in the pathogenesis of cervical cancer.
Annotations
External Annotation for HOTAIR |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
