Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieEndometrial Cancer
CeRNA1HOTAIR[LncRNA]
miRNAmiR-646[miRNA]
CeRNA2NPM1[mRNA]
Tissue/Cell lineHec-1-A And Ishikawa
SpecieHomo sapiens (human)
CitationAm J Physiol Cell Physiol 2018 Jun 1 314, C690-c701 doi:10.1152/ajpcell.00222.2017 PMID:29466670
Reference title
Long non-coding RNA HOTAIR mediates the estrogen-induced metastasis of endometrial cancer cells via miR-646/NPM1 axis.
Experimental verification
qRT-PCR,Western blot,Luciferase reporter assay,in vitro knockdown,RIP
Functional description
MiR-646 expression was significantly decreased both in human EC tissues and cell lines compared with the control.Moreover, miR-646 expression was negatively related to HOTAIR in human EC tissues.miR-646 overexpression considerably attenuated the E2-promoted viability, migration and invasion of Ishikawa and HEC-1-A cells in vitro.In addition, HOTAIR was confirmed to regulate the viability, migration and invasion of EC cells through negative regulating miR-646.More importantly, we also demonstrated that NPM1 was the target of miR-646,and HOTAIR promoted NPM1 expression through interacting with miR-646 in EC cells.HOTAIR was well documented in recruiting PRC2 components and subsequent chromosomal gene silencing via coordinated histone H3K27 methylation and H3K4 demethylation.We therefore performed RNA pull-down assays in Ishikawa cells by means of transfection of the biotin-labelled miR-646 and its mutation and observed that HOTAIR was pulled down by miR-646 but not by miR-646 mutation, suggesting a direct interaction between HOTAIR and miR-646.
Annotations
External Annotation for HOTAIR |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
