Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieGlioblastoma
CeRNA1MCM3AP-AS1[LncRNA]
miRNAmiR-211[miRNA]
CeRNA2KLF5[mRNA]
Tissue/Cell lineHek293T Cells
SpecieHomo sapiens (human)
CitationFront Mol Neurosci 2017 10, 437 doi:10.3389/fnmol.2017.00437 PMID:29375300
Reference title
The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.
Experimental verification
qRT-PCR,Western blot,Luciferase reporter assay,in vitro knockdown,RIP
Functional description
lncRNA antisense 1 to Micro-chromosome maintenance protein 3-associated protein (MCM3AP-AS1) was upregulated whereas miR-211 was downregulated in glioma-associated endothelial cells (GECs). Furthermore, knockdown of MCM3AP-AS1 increased the expression of miR-211.miR-211 targeted KLF5 3'-UTR and consequently inhibited KLF5 expression. Besides,in this study we found that MCM3AP-AS1 knockdown decreased KLF5 and AGGF1 expression by upregulating miR-211.In addition, KLF5 was associated with the promoter region of AGGF1.Knockdown of KLF5 decreased AGGF1 expression by transcriptional repression, and also inhibited the activation of PI3K/AKT and ERK1/2 signaling pathways.
Annotations
External Annotation for MCM3AP-AS1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
