Basic Information
Regular Relationship :
Tissue/Cell lineAgs, Nci-N87, Sgc-7901, Mkn-45, Tmk-1 And Ges-1
SpecieHomo sapiens (human)
CitationOncol Rep 2018 Jul 40, 39-48 doi:10.3892/or.2018.6424 PMID:29749532
Reference title
MEG3/miR-21 axis affects cell mobility by suppressing epithelialmesenchymal transition in gastric cancer.
Experimental verification
qPCR,Western blot,RNAi etc
Functional description
MEG3 was downregulated while miR21 was upregulated in gastric cancer tissues and cell lines by qRT-PCR.Overexpression of MEG3 suppressed cell mobility of gastric cancer cells (AGS) by downregulating the expression of MMP3, MMP9 and VEGF.overexpression of MEG3 also suppressed epithelialmesenchymal transition (EMT) by increasing the expression of an epithelial marker (Ecadherin) and downregulating the expression of mesenchymal markers,indicating that MEG3 suppressed cell mobility through the inhibition of EMT in gastric cancer.The expression of miR21 was negatively regulated by MEG3 and overexpression of miR21 promoted cell mobility of AGS through activation of EMT.Cotransfection of lncRNAMEG3 and miR21 mimic counteracted the inhibitory effect on the cell mobility attributed to MEG3,suggesting that the MEG3/miR21 axis affects cell mobility by suppressing EMT in gastric cancer. Using a mouse xenograft tumor model,we found that the overexpression of MEG3 suppressed tumor growth and metastasis while overexpression of miR21 had the opposite effects.The MEG3/miR21 axis affected gastric cancer growth and metastasis through inhibition of EMT in vivo.In conclusion,we demonstrated that the MEG3/miR21 axis participates in the tumor progression and metastasis of gastric cancer through the regulation of EMT.
Annotations
External Annotation for MEG3 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
