Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieRhabdomyosarcoma
CeRNA1MYCNOS-01[LncRNA]
miRNAmiR-485-5p[miRNA]
CeRNA2MYCN[mRNA]
Tissue/Cell lineRms-01, Cell Line
SpecieHomo sapiens (human)
CitationBMC Cancer 2018 Feb 21 18, 217 doi:10.1186/s12885-018-4129-8 PMID:29466962
Reference title
The long non-coding RNA MYCNOS-01 regulates MYCN protein levels and affects growth of MYCN-amplified rhabdomyosarcoma and neuroblastoma cells.
Experimental verification
Microarray,qRT-PCR,Western blot,in vitro knockdown
Functional description
MYCNOS-01 transcript levels were generally higher in NB and RMS tumor samples and cell lines with MYCN genomic amplification.RNA interference of MYCNOS-01 expression did not alter MYCN transcript levels but decreased MYCN protein levels.Conversely, MYCN reduction increased MYCNOS-01 transcript levels,creating a negative feedback loop on MYCN protein levels.Reduction of MYCNOS-01 or MYCN expression decreased cell growth in MYCN-amplified alveolar rhabdomyosarcoma and neuroblastoma cell lines.This is consistent with MYCNOS-01-mediated regulation of MYCN contributing to the phenotype observed.Another possibility therefore is that MYCNOS-01 interacts with an miRNA that targets MYCN for degradation, therefore increasing MYCN protein expression by sequestering away a negative regulating factor.
Annotations
External Annotation for MYCNOS-01 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
