Osteosarcoma

NEAT1[LncRNA]

miR-34c[miRNA]

BCL-2[mRNA]

Hfob1.19, Mg63, 143B, Hos And Saos2

Homo sapiens (human)

Biosci Rep 2018 Jun 29 38 doi:10.1042/bsr20180375 PMID:29654165

Knockdown of the oncogene LncRNA NEAT1 restores the availability of miR-34c and improves the sensitivity to cisplatin in osteosarcoma.

qPCR,RNAi etc

NEAT1 was overexpressed in OS tissues, which positively correlated with tumor size, Enneking stage and distant metastasis of OS patients. The elevated level of NEAT1 was confirmed in OS cell lines including MG63 and HOS in vitro Knockdown of NEAT1 by two siRNAs induced impaired cell vitalities,promotes the apoptosis and G0/G1 arrest in two cell lines,which was associated with inhibited anti-apoptosis signals BCL-2 pathway and cell cycle-related cyclin D1 (CCND1) signals.Moreover, the tumor suppressor microRNA-34c (miR-34c) was negatively regulated and inhibited by NEAT1 in OS. Suppression of miR-34c could up-regulate the expressions of its target genes BCL-2 and CCND1 to antagonize the effects of NEAT1 knockdown. Furthermore, overexpressed NEAT1 reduced the sensitivity of cisplatin (DDP) and inhibited DDP-induced apoptosis and cell cycle arrest via miR-34c. The results in vivo also confirmed that knockdown of NEAT1 sensitized the OS cells to DPP-induced tumor regression, delayed the tumor growth with reduced levels of Ki-67, BCL-2 and cyclin D1 signals, suggesting that NEAT1 is an oncogene and chemotherapy resistant factor in osteosarcoma.