Colon Cancer

lincRNA-ROR[LncRNA]

miR-145[miRNA]

Sox2[mRNA]

Hb56, Hb96,Tscc, Tca8113, Scc-9 And Cal27, Hek-293T,Oral Cancer Tissues

Homo sapiens (human)

Zhonghua Bing Li Xue Za Zhi 2018 Apr 8 47, 284-290 doi:10.3760/cma.j.issn.0529-5807.2018.04.011 PMID:29690669

LincRNA-ROR functions as a ceRNA to regulate Oct4, Sox2, and Nanog expression by sponging miR-145 and its effect on biologic characteristics of colonic cancer stem cells

qPCR,Western blot,luciferase reporter assay etc

LincRNA-ROR was frequently up-regulated and inversely correlated with miR-145 down-regulation in the colon cancer specimens.LincRNA-ROR was related to tumor size, lymph node involvement and distant metastasis,and miR-145 was found related to tumor size and tumor location.CD44(+) CD133(+) cells were successfully isolated from SW1116 by flow cytometry. The levels of CD44, CD133, Oct4, Sox2, Nanog, lincRNA-ROR in CD44(+) CD133(+) cells were significantly increased,while miR-145 was decreased compared with CD44(-)CD133(-)cells(P<0.05).The levels of CD44,CD133,lnc-ROR in CD44(+) CD133(+) cells were significantly reduced upon cell adherence,while miR-145 was significantly increased.Bioinformatics analysis revealed that lincRNA-ROR shared miRNA response elements with core transcription factors Oct4,Sox2 and Nanog.MiR-145 significantly inhibited the expression of lincRNA-ROR, Oct4, Sox2 and Nanog. Silencing lincRNA-ROR significantly inhibited colon cancer stem cells proliferation and increased the sensitivity to chemotherapy.Linc-ROR functions as a key ceRNA to prevent core TFs,e.g.Oct4, Sox2, Nanog, from miR-145-mediated suppression in colon cancer stem cells and regulates cell proliferation and chemosensitivity.The data may provide insights into the pathophysiological interactions of the components of genetic networks in the development of colon cancer and may lead to new therapies in the future.