Basic Information
Regular Relationship :
Tissue/Cell lineHeb, And Human Gbm Cell Lines
SpecieHomo sapiens (human)
CitationBiochem Biophys Res Commun 2018 Feb 5 496, 712-718 doi:10.1016/j.bbrc.2018.01.109 PMID:29360452
Reference title
Long noncoding RNA SNHG7 promotes the progression and growth of glioblastoma via inhibition of miR-5095.
Experimental verification
Microarray,qRT-PCR,Western blot,Luciferase reporter assay,in vitro knockdown
Functional description
the expression of SNHG7 was significantly upregulated in GBM tissues and cell lines compared with non-cancerous brain tissues. Furthermore,SNHG7 knockdown remarkably suppressed the proliferation, migration and invasion of A172 and U87cells while inducing their apoptosis. Subsequently,SNHG7 knockdown significantly inhibited tumor growth and metastasis invivo by using xenograft experiments in nude mice.SNHG7 directly inhibited miR-5095,which targeted the 3'UTR of CTNNB1 mRNA and subsequently downregulated the Wnt/β-catenin signaling pathway in GBM.Using rescue experiments, we demonstrated that SNHG7 promoted the proliferation, migration and invasion of GBM cells through the inhibition of miR-5095 and concomitant activation of Wnt/β-catenin signaling pathway.miR-5095 mimics inhibited the luciferase activity of A172 and U87 cells transduced with wild type SNHG7 plasmid,while mutations of the putative miR-5095 binding sites in SNHG7 abrogated this effect
Annotations
External Annotation for SNHG7 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
