Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieAcute Myeloid Leukemia
CeRNA1UCA1[LncRNA]
miRNAmiR-125a[miRNA]
CeRNA2HK2[mRNA]
Tissue/Cell lineHl60 And Hs-5
SpecieHomo sapiens (human)
CitationJ Cell Biochem 2018 Jul 119, 6296-6308 doi:10.1002/jcb.26899 PMID:29663500
Reference title
Knockdown of LncRNA-UCA1 suppresses chemoresistance of pediatric AML by inhibiting glycolysis through the microRNA-125a/hexokinase 2 pathway.
Experimental verification
qPCR,Western blot,Luciferase reporter assay,RIP etc
Functional description
UCA1 expression was upregulated following ADR-based chemotherapy.Knockdown of UCA1 increased the cytotoxic effect of ADR and inhibited HIF-1α-dependent glycolysis in ADR-resistant AML cells. Additionally, UCA1 functioned as a ceRNA of miR-125a by directly binding to miR-125a. HK2,a target of miR-125a,was positively regulated by UCA1 in HL60 and HL60/ADR cells.More notably, UCA1 overexpression overturned miR-125-mediated inhibition on HIF-1α-dependent glycolysis in HL60 and HL60/ADR cells.Furthermore, 2-deoxy-glucose (2-DG) exposure inhibited HIF-1α-dependent glycolysis,and attenuated UCA1-induced increase of chemoresistance in HL60 and HL60/ADR cells.We conclude that knockdown of UCA1 plays a positive role in overcoming the chemoresistance of pediatric AML,through suppressing glycolysis by the miR-125a/HK2 pathway, contributing to a better understanding of the molecular mechanism of chemoresistance in AML.
Annotations
External Annotation for UCA1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
