Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieHepatocellular Carcinoma
CeRNA1XIST[LncRNA]
miRNAmiR-194-5p[miRNA]
CeRNA2MAPK1[mRNA]
Tissue/Cell lineMhcc97L, Mhcc97H, Hepg2, Smmc7221, Huh7, Bel-7402
SpecieHomo sapiens (human)
CitationJ Cell Biochem 2018 Jun 119, 4458-4468 doi:10.1002/jcb.26540 PMID:29227532
Reference title
LncRNA XIST functions as a molecular sponge of miR-194-5p to regulate MAPK1 expression in hepatocellular carcinoma cell.
Experimental verification
qPCR,Western blot,Luciferase reporter assay etc
Functional description
XIST was significantly upregulated in HCC tissues and associated with tumor size and vascular invasion.Gain- and loss-of-function of XIST further presented that XIST promoted the progression of HCC cells, including proliferation, migration,and invasion. Moreover, silencing of XIST could inhibit tumor growth in vivo.XIST could target miR-194-5p and thus decrease miR-194-5p expression.Besides that,restoring XIST could reverse the inhibitory effect of miR-194-5p on the proliferation and invasion of HCC cells.We further elucidated such rescue role might through derepressing MAPK1 expression,the target of miR-194-5p.We pinpointed that LncRNA XIST can act as a ceRNA for MAPK1 mRNA through miR-194-5p. These results deepen our understanding of the role of XIST in HCC.
Annotations
External Annotation for XIST |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
