Basic Information
Regular Relationship :
Phenotype/DiseaseSpeciePancreatic Cancer
CeRNA1XIST[LncRNA]
miRNAmiR-34a-5p[miRNA]
CeRNA2NA[mRNA]
Tissue/Cell linePanc-1, Aspc-1, Mia Paca-2,Hpac, Cfapc-1,Bxpc-3,Pc Tissues
SpecieHomo sapiens (human)
CitationOncol Rep 2018 Apr 39, 1591-1600 doi:10.3892/or.2018.6245 PMID:29393501
Reference title
Long non-coding RNA XIST exerts oncogenic functions in pancreatic cancer via miR-34a-5p.
Experimental verification
qPCR,RNAi,in vitro knockdown,Luciferase reporter assay etc
Functional description
Overexpression of XIST significantly promoted the proliferation,migration and invasion, and suppressed cell apoptosis of BxPC-3 cells;knockdown of XIST significantly inhibited the proliferation,migration and invasion,and accelerated cell apoptosis of PANC-1 cells. Furthermore, BxPC-3 and PANC-1 cells transfected with different vectors were injected subcutaneously into nude mice to explore tumor formation. We found that XIST promoted tumor formation in vivo.Subsequently, we found that microRNA-34a-5p (miR34a-5p) was downregulated in PC tissues,and predicted a poor prognosis in PC patients. In addition, the results indicated that miR-34a-5p is a target gene of XIST and was significantly negatively correlated with XIST. More importantly,miR-34a-5p rescued the facilitation of malignant behavior mediated by XIST. These results indicated that XIST and miR-34a-5p may be potential effective therapeutic targets for PC.
Annotations
External Annotation for XIST |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
