Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieChondrosarcoma
CeRNA1HOTAIR[LncRNA]
miRNAmiR-454-3p[miRNA]
CeRNA2STAT3[mRNA]
Tissue/Cell lineHc-A, Sw1353, Oums-27, Hcs-2/8, Jj012
SpecieHomo sapiens (human)
CitationCell Death Dis 2017 Feb 9 8, e2605 doi:10.1038/cddis.2017.31 PMID:28182000
Reference title
Knockdown of long non-coding RNA HOTAIR increases miR-454-3p by targeting Stat3 and Atg12 to inhibit chondrosarcoma growth.
Experimental verification
qPCR,RNAi,Western blot,Luciferase reporter assay,CCK-8 assay etc.
Functional description
HOTAIR expression was upregulated in chondrosarcoma tissues and cell lines. Functional experiments reveal that HOTAIR knockdown leads to growth inhibition of human chondrosarcoma cells in vitro and in vivo. In addition to cycle arrest and apoptosis, knockdown of HOTAIR inhibits autophagy, which favors cell death. Mechanistically, we demonstrated that HOTAIR induced DNA methylation of miR-454-3p by recruiting EZH2 and DNMT1 to the miR-454-3p promoter regions,which markedly silences miR-454-3p expression. Further analysis revealed that STAT3 and ATG12 are targets of miR-454-3p,initiate HOTAIR deficiency-induced apoptosis and reduce autophagy.
Annotations
External Annotation for HOTAIR |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
