Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieNA
CeRNA1anti-miR-204[Artifically engineered RNA]
miRNAmiR-204[miRNA]
CeRNA2Runx2[mRNA]
Tissue/Cell lineMesenchymal Progenitor Cells And Bone Marrow Stromal Cell (Bmsc)
SpecieHomo sapiens (human)
CitationStem Cells 2010 Feb 28, 357-64 doi:10.1002/stem.288 PMID:20039258
Reference title
MicroRNA-204 regulates Runx2 protein expression and mesenchymal progenitor cell differentiation
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
Retroviral overexpression of miR-204 or transfection of miR-204 oligo decreased Runx2 proteinlevels and miR-204 inhibition significantly elevated Runx2 protein levels, suggesting that miR-204 acts as an endogenous attenuator of Runx2in mesenchymal progenitor cells and BMSCs. Mutations of putative miR-204 binding sites upregulated the Runx2 3-UTR reporter activity, suggesting that miR-204/211 bind to Runx2 3-UTR. Perturbation of miR-204 resulted in altered differentiation fate of mesenchymal progenitorcells and BMSCs: osteoblast differentiation was inhibited and adipocyte differentiation was promoted when miR-204 was overexpressed in these cells, where asosteogenesis was upregulated and adipocyte formation was impaired when miR-204 was inhibited.
Annotations
External Annotation for anti-miR-204 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
