Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieRetina
CeRNA1mir-182 sponge[Artifically engineered RNA]
miRNAmiR-182[miRNA]
CeRNA2Slc1a1[mRNA]
Tissue/Cell lineMouse Retina
SpecieHomo sapiens (human)
CitationCell 2010 May 14 141, 618-31 doi:10.1016/j.cell.2010.03.039 PMID:20478254
Reference title
Characterizing light-regulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
To get further support for the role of the miR-183/96/182 cluster in regulating Slc1a1 mRNA, we generated EGFP constructs expressing miR-183/96/182-speci c ‘‘sponges’’ in their 30 -UTR (Ebert et al., 2007). Three sponges, each containing eight sites complementary to one of the three miR-183/96/182 cluster miRNAs and a sponge containing four sites speci c to each of the three miRNAs (triple sponge) were constructed. When tested in mouse NIH 3T3 cells, all sponges markedly relieved the repression of FL-Slc1a1_wt reporter induced by the cotransfection of either single miR-183/ 96/182 cluster miRNAs or a mixture of the three
Annotations
External Annotation for mir-182 sponge |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
