Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieLymphocytic Leukemias
CeRNA1pH1ant-miR-20a[Artifically engineered RNA]
miRNAmiR-20a[miRNA]
CeRNA2E2F1[mRNA]
Tissue/Cell lineK562 Cells
SpecieHomo sapiens (human)
CitationNucleic Acids Res 2007 35, e149 doi:10.1093/nar/gkm971 PMID:18025036
Reference title
Lentivirus-mediated antagomir expression for specific inhibition of miRNA function
Experimental verification
Western blot
Functional description
Here, we describe the induction of stable loss-of-function phenotypes for specific miRNAs by lentivirus-mediated antagomirexpression. Lentivirally expressed antagomirs are transcribed from a H1-promoter located within the lentiviral 3LTR and were directed against miRNAs encoded on the polycistronic miR17-92 transcript. Functional silencing of miR-18a, miR-19b and miR-20a by the corresponding antagomirs specifically relieves miRNA-mediated reporter gene repression. Inhibition of miRNA function correlates to reduction of miRNA amplification by miRNA-specific quantitative RT-PCR. Furthermore, protein expression of E2F1, a known miR-20 target, is enhanced by lentivirally expressed anti-miR-20 antagomirs in a dose-dependent manner, whereas over-expression of miR-20a reduces E2F1 levels.
Annotations
External Annotation for pH1ant-miR-20a |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
