Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieNon-Small Cell Lung Cancer
CeRNA1XIST[LncRNA]
miRNAmiR-449a[miRNA]
CeRNA2BCL-2[mRNA]
Tissue/Cell lineNl9980, Nci-H1299, Nci-H460, Spc-A-1, And A549
SpecieHomo sapiens (human)
CitationActa Pharmacol Sin 2017 Mar 38, 371-381 doi:10.1038/aps.2016.133 PMID:28248928
Reference title
The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer.
Experimental verification
qPCR,RNAi,Western blot,Cell migration and invasion assay,CCK-8 assay etc.
Functional description
The expression levels of XIST were significantly elevated. Knockdown of XIST significantly inhibited the cell proliferation, migration and invasion, and promoted cell apoptosis. Furthermore, XIST knockdown elevated the expression of E-cadherin, and suppressed the expression of Bcl-2. We further identified reciprocal repression between XIST and miR-449a, which eventually influenced the expression of Bcl-2: XIST functioned as a miRNA sponge of miR-449a, which was a negative regulator of Bcl-2.
Annotations
External Annotation for XIST |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
