Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieProstate Cancer
CeRNA1PTENP1[Pseudogene]
miRNAmiR-19b[miRNA]
CeRNA2PTEN[mRNA]
Tissue/Cell line293T , Pc3,Rwpe-1, Pwr-1E And Vcap
SpecieMus musculus (mouse)
CitationNature 2010 Jun 24 465, 1033-8 doi:10.1038/nature09144 PMID:20577206
Reference title
A coding-independent function of gene and pseudogene mRNAs regulates tumour biology
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
We found perfectly conserved seed matches for the PTEN-targeting miR-17, miR-21, miR-214, miR-19 and miR-26 families. To measure the role of these microRNAs on both PTEN and PTENP1 expression, we designed specific PCR primer sets in the non-homologous 3’UTR regions. In DU145 prostate cancer cells, PTEN-targeting microRNAs miR-19b and miR-20a suppress both PTEN and PTENP1 mRNA abundance. In these cells, a pool of inhibitors of endogenously expressed PTEN-targeting microRNAs derepressed both PTEN and PTENP1 transcript levels. The use of chimeric luciferase plasmids indicated the microRNA:PTENP1 interaction was direct. These data indicate that PTENP1 and PTEN are subjected to the same microRNA-mediated, post-transcriptional regulation.
Annotations
External Annotation for PTENP1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
