Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieGastric Cancer
CeRNA1FER1L4[LncRNA]
miRNAmiR-106a-5p[miRNA]
CeRNA2RB1[mRNA]
Tissue/Cell lineGes-1, Ags, Mgc-803, Sgc-7901 Etc.
SpecieHomo sapiens (human)
CitationSci Rep 2014 Aug 15 4, 6088 doi:10.1038/srep06088 PMID:25124853
Reference title
Long noncoding RNA associated-competing endogenous RNAs in gastric cancer.
Experimental verification
microarray, qPCR, RNAi etc.
Functional description
RB1 is one of miR-106a-5p's targets, and has been validated by dual luciferase reporter assay25. In this study, the interaction between FER1L4 and miR-106a-5p was first predicted by miRcode. Then dual luciferase reporter assay showed that the luciferase activity of the mutant FER1L4 plasmid was about 56% higher than that of the wild-type plasmid. This indicated that the mutations introduced in the seed matches impair the ability of miR-106a-5p to bind to FER1L4. qRT-PCR analysis revealed that transfection of small interfering RNA (siRNA) against FER1L4 not only reduced FER1L4 levels in GES-1, AGS, MGC-803 and SGC-7901, but also reduced RB1 levels in all cells tested.
Annotations
External Annotation for FER1L4 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
