Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieCervical Cancer
CeRNA1MALAT1[LncRNA]
miRNAmiR-375[miRNA]
CeRNA2DNMT1[mRNA]
Tissue/Cell lineSiha And Caski
SpecieHomo sapiens (human)
CitationPLoS One 2016 11, e0163460 doi:10.1371/journal.pone.0163460 PMID:27658300
Reference title
MiR-375 Is Epigenetically Downregulated by HPV-16 E6 Mediated DNMT1 Upregulation and Modulates EMT of Cervical Cancer Cells by Suppressing lncRNA MALAT1.
Experimental verification
qPCR, Cell transfection, Western blot, Dual-luciferase reporter assay, Flow cytometry assay etc.
Functional description
MiR-375 overexpression or MALAT1 siRNA partly restored E-cadherin expression and also significantly reduced N-cadherin. Following immunofluorescent staining confirmed these trends. Since EMT is an important mechanism of enhanced tumor cell invasion and metastasis, we further detected how miR-375 and MALAT1 modulate invasion capacity of SiHa cells. Transwell assay showed that both miR-375 overexpression and MALAT1 knockdown significantly reduced invasion capacity of SiHa cells.
Annotations
External Annotation for MALAT1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
