Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieHistiocytic Lymphoma
CeRNA1SFFV-142[Artifically engineered RNA]
miRNAmiR-142-3p[miRNA]
CeRNA2NA[mRNA]
Tissue/Cell lineU937 Cells
SpecieMus musculus (mouse)
CitationNat Methods 2009 Jan 6, 63-6 doi:10.1038/nmeth.1277 PMID:19043411
Reference title
Stable knockdown of microRNA in vivo by lentiviral vectors
Experimental verification
Western blot
Functional description
In PGK-223T–transduced cells, we observed lower GFP fluorescence for all vector copy numbers tested, indicating thatmiR-223 was regulating its target. Next we replaced the PGK1 promoter with the stronger spleen focus forming virus enhancer/promoter (SFFV) in both vectors, and transduced U937 cells with the constructs, which we named SFFV-GFP and SFFV-223T. Again, uorescence increased proportionally with the number of integrated vectors
Annotations
External Annotation for SFFV-142 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
