Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieBreast Cancer
CeRNA1XIST[LncRNA]
miRNAmiR-125b-5p[miRNA]
CeRNA2NLRC5[mRNA]
Tissue/Cell lineThree breast cancer cell lines (MCF-7, MDA-MB-231 and SKBR3 cells) and normal breast epithelial cell line (MCF-10A cells)
SpecieHomo sapiens (human)
CitationAm J Transl Res 2020 12, 3501-3511, PMID:32774715
Reference title
The lncRNA XIST promotes the progression of breast cancer by sponging miR-125b-5p to modulate NLRC5.
Experimental verification
qRT-PCR,Western blot,luciferase reporter assay,Flow cytometry etc.
Functional description
Both XIST (a lncRNA) and NLRC5 are overexpressed, and miR-125b-5p is downregulated, in breast cancer tissues and cells. XIST suppression decreased cell growth, cell metastasis, and anti-apoptosis activity. Both XIST and NLRC5 contain binding sites for miR-125b-5p, as initially predicted in our study. Decreasing XIST levels increased miR-125b-5p expression, and the upregulation of miR-125b-5p produced similar inhibitory effects on breast cancer cells as that mediated by the loss of XIST.
Annotations
External Annotation for XIST |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
