Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieMultiple Sclerosis
CeRNA1LV-ets-1-3UTR-MRE[Artifically engineered RNA]
miRNAmiR-326[miRNA]
CeRNA2Ets1[mRNA]
Tissue/Cell lineHek293T,Th Cells
SpecieMus musculus (mouse)
CitationNat Immunol 2009 Dec 10, 1252-9 doi:10.1038/ni.1798 PMID:19838199
Reference title
MicroRNA miR-326 regulates TH-17 differentiation and is associated with the pathogenesis of multiple sclerosis
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
The braodly used microRNA inhibitor LNA-anti-miR-326 (a locked nucleic acid– modified antisense oligonucleotide directed against miR-326) pro- duced an inhibitory effect on TH-17 differentiation similar to that of infection with LV-sponge. We also differentiated TH-17 cells in vitro with a bicistronic retrovirus encoding miR-326, an internal ribosomal entry site and GFP. Relative to infection by control retrovi- rus (encoding mutant miR-326), transduction of miR-326-encoding virus resulted in a substantially higher percentage of IL-17-expressing cells in the GFP+ population, whereas infection with virus encoding a specific inhibitor of miR-326 resulted in a much lower percentage of IL-17-expressing GFP+ cells.
Annotations
External Annotation for LV-ets-1-3UTR-MRE |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
