Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieRetinoblastoma
CeRNA1SNHG20[LncRNA]
miRNAmiR-335-5p[miRNA]
CeRNA2E2F3[mRNA]
Tissue/Cell lineRB tissues and cell lines
SpecieHomo sapiens (human)
CitationMol Med Rep 2021 Aug 24 doi:10.3892/mmr.2021.12182 PMID:34080033
Reference title
Long non-coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR-335-5p/E2F3 axis
Experimental verification
qRT-PCR
Functional description
The impact of SNHG20 status on cell proliferation, survival, migration and invasion was determined using small interfering RNA and a range of established experimental assays. The SNHG20/microRNA (miR)-335-5p/E2F transcription factor 3 (E2F3) signaling axis was further investigated using a dual-luciferase activity reporter system and an RNA pull-down assay combined with bioinformatics analyses. SNHG20 expression was significantly increased in RB tissues and cell lines. Silencing of SNHG20 in RB cells was shown to inhibit cell proliferation, clonogenic survival, migration and invasion. Moreover, mechanistic investigations demonstrated that SNHG20 could enhance the expression of E2F3 by sponging of miR-335-5p. These data suggested that the long non-coding RNA SNHG20 may promote cell proliferation, migration and invasion in RB via the miR-335-5p/E2F3 axis.
Annotations
External Annotation for SNHG20 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
