Oral Squamous Cell Cancer

LSINCT5[LncRNA]

miR-185-5p[miRNA]

ZNF703[mRNA]

OSCC cells

Homo sapiens (human)

J BUON. 2021 Jan-Feb;26(1):124-131.

LncRNA LSINCT5 drives proliferation and migration of oral squamous cell carcinoma through the miRNA-185-5p/ZNF703 axis.

Dual-luciferase reporter assay;qRT-PCR;Luciferase reporter assay;

PURPOSE: To analyze the role of lncRNA LSINCT5 in oral squamous cell carcinoma (OSCC) progression and the molecular mechanism. METHODS: QRT-PCR was conducted to detect LSINCT5 levels in OSCC tissues and cell lines. Survival analysis in OSCC patients based on LSINCT5 levels was performed using Kaplan-Meier method. Influence of LSINCT5 on functions of OSCC cells were assessed by CCK-8, EdU and Transwell assay. Bioinformatic analysis and dual-luciferase reporter assay were carried out to identify the interaction between LSINCT5 and the miRNA (miR)-185-5p/zNF703 axis. Rescue experiments were conducted to uncover the molecular mechanism of LSINCT5 in regulating OSCC cell functions. RESULTS: LSINCT5 was upregulated in OSCC specimens and correlated to poor prognosis of OSCC. Knockdown of LSINCT5 inhibited proliferative and migratory capacities of OSCC. LSINCT5 could target and negatively regulate miR-185-5p. Moreover, miR-185-5p was the key downstream gene that was responsible for the carcinogenic role of LSINCT5 in regulating OSCC cell functions. The oncogenic gene ZNF703 was proven to be the target binding miR-185-5p. CONCLUSIONS: LSINCT5 is abnormally upregulated in OSCC specimens and drives its malignant progression through the miR-185-5p/ZNF703 axis.