Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieMycoplasma Pneumoniae Pneumonia
CeRNA1GAS5[LncRNA]
miRNAmiR-222-3p[miRNA]
CeRNA2TIMP3[mRNA]
Tissue/Cell lineMPP cells
SpecieHomo sapiens (human)
CitationMol Med Rep. 2021 May;23(5):380. doi: 10.3892/mmr.2021.12019. Epub 2021 Mar 24.
Reference title
Long non-coding RNA GAS5 protects against Mycoplasma pneumoniae pneumonia by regulating the microRNA-222-3p/TIMP3 axis.
Experimental verification
Dual-luciferase reporter assay;ELISA;MTT assay;Luciferase reporter assay;MTT assay;
Functional description
Mycoplasma pneumoniae pneumonia (MPP) is a type of pneumonia induced by M. pneumoniae (MP) infection. The present study investigated the effect of long non-coding RNA growth arrest-specific 5 (GAS5) in MPP and the underlying molecular mechanism of this. The expression of GAS5, microRNA-222-3p, (miR-222-3p) and tissue inhibitor of metalloproteinases-3 (TIMP3) in MPP was investigated using reverse transcription-quantitative PCR. Lipid-associated membrane protein (LAMP)-induced THP-1 cells were used to model MPP. The viability of LAMP-induced THP-1 cells was analyzed using an MTT assay. Expression levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) pro-inflammatory cytokines, and the anti-inflammatory cytokine heme oxygenase-1 (HO-1) in LAMP-induced THP-1 cells were measured by ELISA. A dual-luciferase reporter assay assessed the associations among GAS5, miR-222-3p and TIMP3. The expression of GAS5 and TIMP3 was downregulated in MPP. Expression of miR-222-3p was upregulated. GAS5-overexpression increased the viability of LAMP-induced THP-1 cells. GAS5 upregulation decreased the levels of IL-1β, IL-6, TNF-α and HO-1 levels in LAMP-induced THP-1 cells. GAS5 directly interacted with miR-222-3p. TIMP3 was a target of miR-222-3p. miR-222-3p upregulation or TIMP3-knockdown reversed the promotion effect on cell viability as well as the inhibitory effect on inflammation caused by GAS5-overexpression in LAMP-induced THP-1 cells. GAS5-overexpression increased the viability and decreased the inflammation of LAMP-induced THP-1 cells by regulating the miR-222-3p/TIMP3 axis. These results demonstrated a potential therapeutic target for MPP treatment.
Annotations
External Annotation for GAS5 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
