Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieOvarian Cancer
CeRNA1OIP5-AS1[LncRNA]
miRNAmiR-128-3p[miRNA]
CeRNA2CCNG1[mRNA]
Tissue/Cell lineOC tissues and cells
SpecieHomo sapiens (human)
CitationMol Med Rep. 2021 May;23(5):388. doi: 10.3892/mmr.2021.12027. Epub 2021 Mar 24.
Reference title
Long non-coding RNA OIP5-AS1 facilitates the progression of ovarian cancer via the miR-128-3p/CCNG1 axis.
Experimental verification
Dual-luciferase reporter assay;RNA immunoprecipitation;RNA pull-down assay;Western blot;Luciferase reporter assay;RNA immunoprecipitation;RNA pull-down;
Functional description
Long non-coding RNA (LncRNA) o-phthalaldehyde-interacting protein 5 antisense transcript 1 (OIP5-AS1) serves major roles in the progression of various types of cancer. The present study investigated its biological function in ovarian cancer (OC) and its mechanisms. The levels of OIP5-AS1, microRNA-128-3p (miR-128-3p) and cyclin G1 (CCNG1) were examined by reverse transcription-quantitative PCR. Cell viability, apoptosis, migration and invasion were detected to analyze cellular progression. Glycolytic metabolism was assessed by detecting the levels of glucose consumption and lactate production. CCNG1 and hexokinase 2 protein levels were measured by western blotting. Dual-luciferase reporter assay, RNA immunoprecipitation and RNA pull-down assays were performed to affirm the interaction between two molecules. OIP5-AS1 was found to be upregulated in OC tissues and cells. Knockdown of OIP5-AS1 suppressed cell viability, migration, invasion and glycolysis while promoting apoptosis in OC cells. OIP5-AS1 interacted with miR-128-3p and functioned as an oncogene by sequestering miR-128-3p. In addition, CCNG1 was a target gene for miR-128-3p and miR-128-3p regulated the CCNG1-induced effects on OC cells by downregulating CCNG1. OIP5-AS1 upregulated the expression of CCNG1 via targeting miR-128-3p. OIP5-AS1 knockdown also inhibited tumor growth of OC in vivo by modulating the expression of miR-128-3p and CCNG1. Collectively, these data illustrated that the oncogenic role of OIP5-AS1 in OC was associated with the miR-128-3p/CCNG1 axis at least in part. OIP5-AS1 might be a probable diagnostic and therapeutic biomarker for the treatment of OC patients.
Annotations
External Annotation for OIP5-AS1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
