Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieOsteogenic Differentiation
CeRNA1IGF2-AS[LncRNA]
miRNAmiR-3126-5p[miRNA]
CeRNA2KLK4[mRNA]
Tissue/Cell linebone marrow mesenchymal stem cells
SpecieHomo sapiens (human)
CitationJ Gene Med. 2021 Jun 7:e3372. doi: 10.1002/jgm.3372.
Reference title
LncRNA IGF2-AS promotes the osteogenic differentiation of bone marrow mesenchymal stem cells by sponging miR-3126-5p to upregulate KLK4.
Experimental verification
Dual-luciferase reporter assay;microarray;qPCR;RT-qPCR;RT-PCR;Western blot;Luciferase reporter assay;
Functional description
BACKGROUND: Osteoporosis (OP) is a bone disease with a reduced amount and quality of bone. This study was designed to explore the role and mechanism of lncRNA IGF2-AS in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). METHODS: Human lncRNA and miRNA microarray analyses were performed to detect the differential expression levels of lncRNAs and miRNAs in undifferentiated and osteogenically differentiated BMSCs. LncRNA IGF2-AS, miR-3126-5p, and KLK4 levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The osteogenic differentiation of BMSCs was assessed by alkaline phosphatase (ALP) staining and Alizarin Red staining (ARS). Protein levels of Osterix (Osx), osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2) were examined by RT-PCR and western blot assays. The binding relationship between miR-3126-5p and lncRNA IGF2-AS or KLK4 was predicted by TargetScan (http://www.targetscan.org/vert_72/) and then verified with a dual-luciferase reporter assay. RESULTS: LncRNA IGF2-AS and KLK4 were highly expressed and miR-3126-5p was weakly expressed in osteogenically differentiated BMSCs. Moreover, lncRNA IGF2-AS overexpression enhanced the osteogenic differentiation of BMSCs. In contrast, lncRNA IGF2-AS knockdown showed the opposite trend. Moreover, miR-3126-5p overexpression abolished the lncRNA IGF2-AS-mediated osteogenic differentiation of BMSCs. LncRNA IGF2-AS functions as a sponge of miR-3126-5p to regulate KLK4 expression. CONCLUSION: LncRNA IGF2-AS enhances the osteogenic differentiation of BMSCs by modulating the miR-3126-5p/KLK4 axis, suggesting a promising therapeutic target for bone-related diseases.
Annotations
External Annotation for IGF2-AS |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
