Alzheimers Disease

BACE1-AS[LncRNA]

miR-214-3p[miRNA]

ATG5[mRNA]

alleviated neuronal injury

Homo sapiens (human)

Neuroscience. 2021 Feb 10;455:52-64. doi: 10.1016/j.neuroscience.2020.10.028. Epub 2020 Nov 13.

LncRNA BACE1-AS Promotes Autophagy-Mediated Neuronal Damage Through The miR-214-3p/ATG5 Signalling Axis In Alzheimer's Disease.

Dual-luciferase reporter assay;qRT-PCR;Western blot;Flow Cytometry assay;Luciferase reporter assay;

Alzheimer's disease (AD) is the most common neurodegenerative disease and is characterized by progressive memory loss and cognitive dysfunction. Long non-coding RNAs (lncRNAs) have been shown to be among the most promising biomarkers and therapeutic targets of AD. Here, we aimed to investigate whether lncRNA BACE1-AS plays a role in the potential mechanisms of AD. The expression of BACE1-AS, miR-214-3p and ATG5 mRNA was detected using qRT-PCR. The expression of the LC3, P62, ATG5, Bcl-2, p-Tau and cleaved-caspase 3 proteins was examined using western blot analysis. Cell apoptosis, cytotoxicity and ROS levels were estimated using flow cytometry, an LDH kit and a DCFH-DA assay, respectively. The interaction between BACE1-AS or ATG5 and miR-214-3p was validated using a dual-luciferase reporter assay. HE staining and a TUNEL assay were employed to evaluate hippocampal neuronal injury. The BACE1-AS level was found to be upregulated in serum samples of AD patients, brain tissues of AD transgenic (Tg) mice and Aβ(1-42)-treated SH-SY5Y cells. Autophagy activity was increased in both Tg mice and Aβ(1-42)-treated cells. BACE1-AS knockdown alleviated Aβ(1-42)-induced cell injury. Rapamycin abolished the protective effects of sh BACE1-AS against Aβ(1-42) induced cell injury. BACE1-AS indirectly regulated ATG5 expression by binding miR-214-3p. The miR-214-3p inhibitor reversed the protective effects of sh BACE1-AS and sh ATG5 against Aβ(1-42)-induced cell injury. Knockdown of BACE1-AS alleviated neuronal injury by repressing autophagy in vivo. Our findings demonstrate that silencing of BACE1-AS alleviated neuronal injury by regulating autophagy through the miR-214-3p/ATG5 signalling axis in AD.