Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieOvarian Cancer
CeRNA1Circ_0025033[Circular RNA]
miRNAmiR-184[miRNA]
CeRNA2LSM4[mRNA]
Tissue/Cell lineOC cells
SpecieHomo sapiens (human)
CitationPathol Res Pract. 2021 Jan;217:153275. doi: 10.1016/j.prp.2020.153275. Epub 2020 Nov 17.
Reference title
Circ_0025033 promotes the progression of ovarian cancer by activating the expression of LSM4 via targeting miR-184.
Experimental verification
Dual-luciferase reporter assay;qRT-PCR;Western blot;Luciferase reporter assay;
Functional description
BACKGROUND: Ovarian cancer (OC) is the leading disorder to threaten women's lives. Numerous circular RNAs (circRNAs) were identified in cancers with dysregulation and involved in the pathogenesis of cancer. This study investigated the function and regulatory mechanism of circ_0025033 in OC development, aiming to provide a potential strategy for OC treatment. METHODS: For expression analysis, the expression levels of circ_0025033, LSM4 mRNA and miR-184 were detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the protein level of LSM4 expression was detected by western blot. For functional analysis, the capacities of colony formation, migration/invasion and glycolysis metabolism were assessed by colony formation assay, transwell assay and the levels of glucose consumption and lactate production. The interaction between miR-184 and circ_0025033 or LSM4 was predicted by the bioinformatics tool and validated by dual-luciferase reporter assay. Xenograft models were established to determine the role of circ_0025033 in vivo. RESULTS: The expression of circ_0025033 and LSM4 was promoted in OC tissues and cells. Circ_0025033 knockdown or LSM4 knockdown blocked the ability of colony formation, migration/invasion and glycolysis metabolism in OC cells. In mechanism, circ_0025033 functioned as a "competing endogenous RNA (ceRNA)" to modulate LSM4 expression by targeting miR-184. LSM4 overexpression recovered the inhibitory effects on colony formation, migration/invasion and glycolysis metabolism caused by circ_0025033 knockdown. Moreover, circ_0025033 knockdown also inhibited tumor growth in vivo by regulating LSM4 and targeting miR-184. CONCLUSION: Circ_0025033 promotes the progression of OC by regulating LSM4 expression via targeting miR-184, which provided a new strategy to treat OC.
Annotations
External Annotation for Circ_0025033 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
