Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieMiddle Cerebral Artery Occlusion
CeRNA1H19[LncRNA]
miRNAmiR-29b[miRNA]
CeRNA2SIRT1[mRNA]
Tissue/Cell lineHT22 cells
SpecieMus musculus (mouse)
CitationMol Med Rep. 2021 Feb;23(2):131. doi: 10.3892/mmr.2020.11770. Epub 2020 Dec 14.
Reference title
Long non-coding RNA H19 inhibition ameliorates oxygen-glucose deprivation-induced cell apoptosis and inflammatory cytokine expression by regulating the microRNA-29b/SIRT1/PGC-1α axis.
Experimental verification
ELISA;qPCR;RT-qPCR;Western blot;Flow Cytometry assay;
Functional description
As one of the earliest discovered long non-coding (lnc)RNAs, lncRNA H19 imprinted maternally expressed transcript (H19) participates in regulating ischemic stroke. The present study aimed to investigate the combined roles of lncRNA H19, microRNA (miR)-29b, silent mating-type information regulation 2 homolog 1 (SIRT1) and peroxisome proliferator-activated receptor-g co-activator-1α (PGC-1α) following ischemic stroke. lncRNA H19 expression levels in the middle cerebral artery occlusion (MCAO) mouse model and HT22 cells subjected to oxygen-glucose deprivation (OGD) were detected via reverse transcription-quantitative PCR (RT-qPCR). H19 small interfering RNA was used to knockdown H19 expression. Following OGD treatment, MTT, flow cytometry, ELISA, RT-qPCR and western blotting assays were performed to assess cell proliferation, cell apoptosis, inflammatory cytokine concentrations, and lncRNA H19, miR-29b, SIRT1, PGC-1α expression levels, respectively. In the present study, MCAO model mice and OGD-treated cells displayed significantly increased lncRNA H19 expression levels compared with sham mice and control cells, respectively. lncRNA H19 knockdown ameliorated OGD-induced cell apoptosis and increases in inflammatory cytokine concentrations. Furthermore, lncRNA H19 knockdown also attenuated OGD-mediated downregulation of miR-29b, SIRT1 and PGC-1α expression levels. Collectively, the results of the present study demonstrated that lncRNA H19 knockdown ameliorated OGD-induced cell apoptosis and increases in inflammatory cytokine concentrations by regulating miR-29b, SIRT1 and PGC-1α expression levels, which suggested the potential role of lncRNA H19 in ischemic stroke.
Annotations
External Annotation for H19 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
