Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieApoptosis Induced By Ischemia-Reperfusion Injury
CeRNA1HOTAIR[LncRNA]
miRNAmiR-130a-3p[miRNA]
CeRNA2MDM4[mRNA]
Tissue/Cell lineH9c2 (rat cardiomyocyte line) cells
SpecieRattus (rat)
CitationMol Med Rep. 2021 Mar;23(3):169. doi: 10.3892/mmr.2020.11808. Epub 2021 Jan 5.
Reference title
Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury.
Experimental verification
Cell transfection;Cell transfection;microarray;RNA immunoprecipitation;Western blot;Flow Cytometry assay;Luciferase reporter assay;RNA immunoprecipitation;
Functional description
The present study aimed to investigate the effect of the long non-coding ribonucleic acid (lncRNA) HOX transcript antisense intergenic RNA (HOTAIR) on apoptosis induced by ischemia-reperfusion injury. Differential lncRNAs in myocardial ischemia rats were screened by a lncRNA microarray and the expression levels of lncRNA HOTAIR and microRNA (miR)-130a-3p were analyzed using reverse transcription-quantitative polymerase chain reaction in hypoxia-induced cardiomyocytes. The mechanism of lncRNA HOTAIR in cardiotoxicity was investigated using cell transfection, lncRNA knockdown, Cell Counting Kit-8, flow cytometry, western blotting, dual luciferase reporter assays and RNA immunoprecipitation. The expression level of lncRNA HOTAIR was significantly downregulated in the ischemic myocardium of rats. Overexpression of HOTAIR in H9c2 (rat cardiomyocyte line) cells could inhibit the apoptosis induced by H2O2. A direct interaction was found between HOTAIR and miR-130a-3p, and mouse double minute 4 (MDM4) was also found to be a potential target of miR-130a-3p. The overexpression of MDM4 in H9c2 cells transfected with miR-130a-3p mimics increased apoptosis, and miR-130a-3p targeted inhibition of MDM4 promoted H2O2-induced apoptosis of H9c2 cells. Overall, HOTAIR was found to inhibit the apoptosis of H9c2 cells induced by H2O2 through the miR-130a-3p/MDM4 axis.
Annotations
External Annotation for HOTAIR |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
