Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieBreast Cancer
CeRNA1FN1[Coding-mRNA]
miRNAmiR-671[miRNA]
CeRNA2CD44[mRNA]
Tissue/Cell lineHuman Breast Carcinoma Cell Line Mda-Mb-231
SpecieHomo sapiens (human)
CitationJ Cell Sci 2012 Apr 15 125, 2075-85 doi:10.1242/jcs100818 PMID:22637644
Reference title
The non-coding 3 UTR of CD44 induces metastasis by regulating extracellular matrix functions
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
Here, we demonstrate that the overexpression of the CD44 3 UTR results in enhanced cell motility, invasion and cell adhesion in human breast carcinoma cell line MDA-MB-231. Furthermore, we found that expression of the CD44 3 UTR enhances metastasis in vivo.Computational analysis indicated that miRNAs that interact with the CD44 3 UTR also have binding sites in other matrix-encoding mRNA 3 UTRs, including collagen type 1a1 (Col1a1) repressed by miR-328 and fibronectin type 1 (FN1) repressed by miR-512-3p, miR-491 and miR-671. Protein analysis demonstrated that expression of CD44, Col1a1 and FN1 were synergistically upregulated in vitro and in vivo upon transfection of the CD44 3 UTR. The non-coding 3 UTR of CD44 interacts with multiple miRNAs that target extracellular matrix properties and thus can be used to antagonize miRNA activities.
Annotations
External Annotation for FN1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
