Non-Small Cell Lung Cancer

PTPRG-AS1[LncRNA]

miR-200c-3p[miRNA]

TCF4[mRNA]

Non small cell lung cancer cells

Homo sapiens (human)

Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820942615. doi: 10.1177/1533033820942615.

Long Noncoding RNA PTPRG Antisense RNA 1 Reduces Radiosensitivity of Non small cell lung cancer Cells Via Regulating MiR-200c-3p/TCF4.

Western blot;Immunohistochemistry;Luciferase reporter assay;

BACKGROUND: PTPRG antisense RNA 1 has been well-documented to exert an oncogenic role in diverse neoplasms. However, the precise role of PTPRG antisense RNA 1 in regulating radiosensitivity of Non small cell lung cancer cells remains largely elusive. METHODS: Expression levels of PTPRG antisense RNA 1 and miR-200c-3p in Non small cell lung cancer tissues and cells were detected by quantitative real-time polymerase chain reaction, while transcription factor 4 expression was examined by immunohistochemistry and Western blot. After Non small cell lung cancer cells were exposed to X-ray with different doses in vitro, Cell Counting Kit-8 assay and colony formation assay were conducted to determine the influence of PTPRG antisense RNA 1 on cell viability. Interaction between miR-200c-3p and PTPRG antisense RNA 1 as well as transcription factor 4 was investigated by dual luciferase reporter assay. RESULT: In Non small cell lung cancer tissues, the expressions of PTPRG antisense RNA 1 and transcription factor 4 were significantly upregulated, whereas the expression of miR-200c-3p was downregulated. It was also proved that PTPRG antisense RNA 1 and 3'-untranslated region of transcription factor 4 can bind to miR-200c-3p. Under X-ray irradiation, overexpressed PTPRG antisense RNA 1 could promote the viability and enhance the radioresistance of Non small cell lung cancer cells, and this effect was partially weakened by miR-200c-3p mimics. Transcription factor 4 was identified as a target gene of miR-200c-3p, which could be positively regulated by PTPRG antisense RNA 1. CONCLUSION: PTPRG antisense RNA 1 reduces the radiosensitivity of Non small cell lung cancer cells via modulating miR-200c-3p/TCF4 axis.