Non-Small Cell Lung Cancer

SNHG10[LncRNA]

miR-21[miRNA]

NA[mRNA]

NSCLC cells

Homo sapiens (human)

BMC Pulm Med. 2020 Oct 20;20(1):273. doi: 10.1186/s12890-020-01281-w.

LncRNA SNHG10 is downregulated in non-small cell lung cancer and predicts poor survival.

CCK-8 assay;qPCR;RT-qPCR;

BACKGROUND: LncRNA SNHG10 has been reported to be an oncogenic lncRNA in liver cancer. However, its roles in non-small cell lung cancer (NSCLC) remains unknown. METHODS: Tumor and paired non-tumor tissues were harvested from 62 NSCLC patients. RT-qPCR was used to detect the expression of SNHG10 and miR-21 in tissues. Overexpression experiments were used to evaluate the interaction between SNHG10 and miR-21 in NSCLC cells. CCK-8 assay was used to detect the cell proliferation. RESULTS: We observed the expression of SNHG10 was down-regulated in non-small cell lung cancer (NSCLC) compared with that in non-tumor tissues. Moreover, we found that high expression levels of SNHG10 predicted favorable survival of NSCLC patients, and the expression of miR-21 were increased in NSCLC and inversely correlated with SNHG10 expression. In NSCLC cells, overexpression of SNHG10 resulted in increased miR-21 gene methylation and decreased miR-21 expression. Moreover, overexpression of SNHG10 attenuated the enhancing effect of miR-21 overexpression on cell proliferation. CONCLUSIONS: SNHG10 may involve in NSCLC cell proliferation by regulating the miR-21 gene methylation.