Breast Cancer

SNHG7[LncRNA]

miR-15a[miRNA]

NA[mRNA]

MCF7 and T47D

Homo sapiens (human)

J BUON. 2020 Jul-Aug;25(4):1792-1798.

LncRNA SNHG7 inhibits proliferation and invasion of breast cancer cells by regulating miR-15a expression.

Cell proliferation assay;Dual-luciferase reporter assay;qRT-PCR;Luciferase reporter assay;

PURPOSE: To explore the inhibition of proliferation and invasion of breast cancer cells by LncRNA SNHG7 via regulating the expression of miR-15a and its mechanism. METHODS: The expression of SNHG7 in breast cancer and adjacent tissues and different breast cancer cells was measured by qRT-PCR and the relationship between SNHG7 and clinicopathological parameters of breast cancer patients was analyzed. The interaction of SNHG7 with miR-15a was explored by dual-luciferase reporter assay. The change in the proliferation of breast cancer cells after silencing SNHG7 was examined by cell proliferation assay. The change in the invasion of breast cancer cells after silencing SNHG7 was examined by Transwell invasion assay. Subcutaneous tumor formation in nude mice was detected to record the tumor size and volume of tumor cells. RESULTS: Compared with adjacent normal tissues, the expression of SNHG7 was significantly increased in breast cancer tissues; the expression of SNHG7 was the highest in breast cancer cells MCF7 and T47D; the expression level of SNHG7 was not notably different among breast cancer patients of different genders and age groups, and the difference was not statistically significant (p>0.05). The expression level of SNHG7 was higher in patients with a higher stage of breast cancer and patients with lymph node metastasis, and the difference was statistically significant. SNHG7 could specifically bind to the 3' UTR of miR-15a. The inhibition of SNHG7 led to constrained proliferation and invasion of breast cancer cells. The tumor volume and weight of the tumor-bearing mice in the si-SNHG7 group were significantly lower than those in the non-specific control (NC) group. CONCLUSION: SNHG7 plays an important role in the development of breast cancer. SNHG7 can affect the proliferation and invasion of breast cancer cells through its targeted regulation of miR-15a activity.