Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieOvarian Cancer
CeRNA1ASAP1-IT1[LncRNA]
miRNAmiR-2278[miRNA]
CeRNA2YAP[mRNA]
Tissue/Cell lineovarian cancer cells
SpecieHomo sapiens (human)
CitationInt J Mol Med. 2021 Apr;47(4):44. doi: 10.3892/ijmm.2021.4877. Epub 2021 Feb 12.
Reference title
Long non-coding RNA ASAP1-IT1 suppresses ovarian cancer progression by regulating Hippo/YAP signaling.
Experimental verification
luciferase assay;
Functional description
Long non-coding RNAs (lncRNAs) are a class of non-protein coding transcripts that are involved in the regulation of gene expression in mammalian cells. Transcriptional co-activator Yes associated protein 1 (YAP1) plays a key role in the progression of ovarian cancer. However, the regulation of Hippo/YAP signaling in ovarian cancer remains elusive. In the present study, the expression levels of lncRNA ASAP1-IT1 were investigated. The analysis indicated that lncRNA ASAP1-IT1 expression was downregulated in ovarian tumor samples and ovarian cancer cells. The overexpression of ASAP1-IT1 inhibited ovarian cancer cell proliferation and induced cell apoptosis. Bioinformatics analysis predicted that miR-2278, a previously reported upregulated miRNA in ovarian tumors, may bind to ASAP1-IT1. Dual luciferase assay confirmed the direct regulatory association between ASAP1-IT1 and miR-2278. In addition, the data demonstrated that large tumor suppressor 2 (LATS2) was a target gene of miR-2278, whose expression was upregulated by ASAP1-IT1 in ovarian cancer cells. By regulating the expression of LATS2, ASAP1-IT1 induced the downregulation of YAP1 expression in ovarian cancer cells. Moreover, the silencing of LATS2 attenuated the inhibition of cell proliferation and the apoptosis induced by ASAP1-IT1 overexpression in ovarian cancer cells. The association among the expression levels of ASAP1-IT1, miR-2278 and LATS2 was observed in specimens obtained from patients with ovarian cancer. Taken together, the data presented herein demonstrate that ASAP1-IT1 functions as a potential tumor suppressor lncRNA by upregulating LATS2 expression in ovarian cancer.
Annotations
External Annotation for ASAP1-IT1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
