Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieMammary Carcinoma
CeRNA1CDC42[Coding-mRNA]
miRNAmiR-608[miRNA]
CeRNA2CD44[mRNA]
Tissue/Cell lineHuman Mammary Carcinoma Cells Mt-1
SpecieHomo sapiens (human)
CitationNucleic Acids Res 2011 Apr 39, 3026-41 doi:10.1093/nar/gkq1003 PMID:21149267
Reference title
Expression of CD44 3 -untranslated region regulates endogenous microRNA functions in tumorigenesis and angiogenesis
Experimental verification
Immunohistochemistry;Luciferase reporter assay;Western blot
Functional description
Computational algorithms have predicted three miRNAs, miR-216a, miR-330 and miR-608, can bind to both the CD44 and CDC42 3-UTRs. This was confirmed with luciferase assays, western blotting and immunohistochemical staining and correlated with a series of siRNA assays. Thus, the non-coding CD44 3-UTR serves as a competitor for miRNA binding and subsequently inactivates miRNA functions, by freeing the target mRNAs from being repressed.
Annotations
External Annotation for CDC42 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
