Parkinsons Disease

LINC00943[LncRNA]

miR-15b-5p[miRNA]

RAB3IP[mRNA]

MPP(+)-challenged SK-N-SH cells

Homo sapiens (human)

Neurol Res. 2021 Mar;43(3):181-190. doi: 10.1080/01616412.2020.1834290. Epub 2020 Nov 19.

LINC00943 knockdown attenuates MPP(+)-induced neuronal damage via miR-15b-5p/RAB3IP axis in SK-N-SH cells.

RNA immunoprecipitation;Western blot;RNA immunoprecipitation;

OBJECTIVES: Parkinson's disease (PD) is a neurodegenerative problem correlated with neuronal damage. Long noncoding RNAs (lncRNAs) are implicated in neuronal damage in PD development. This research aims to analyze the function and mechanism of LINC00943 in 1-methyl-4-phenylpyridinium (MPP(+))-caused neuronal injury. METHODS: MPP(+)-challenged SK-N-SH cells served as a PD-like model of neuronal damage. LINC00943, microRNA-15b-5p (miR-15b-5p) and RAB3A interacting protein (RAB3IP) abundances were examined via quantitative reverse transcription polymerase chain reaction or western blot. MPP(+)-caused neuronal damage was assessed via cell viability, apoptosis, inflammatory injury and oxidative injury. The association between miR-15b-5p and LINC00943 or RAB3IP was determined via dual-luciferase reporter analysis and RNA immunoprecipitation. RESULTS: LINC00943 abundance was up-regulated in MPP(+)-challenged SK-N-SH cells. LINC00943 silence alleviated MPP(+)-caused decrease of cell viability and elevation of apoptosis, inflammatory injury and oxidative injury. miR-15b-5p was inhibited via LINC00943, and miR-15b-5p inhibition reversed knockdown of LINC00943-mediated suppression of MPP(+)-induced neuronal damage. RAB3IP was targeted via miR-15b-5p, and LINC00943 could regulate RAB3IP via miR-15b-5p. miR-15b-5p addition mitigated MPP(+)-induced neuronal damage through decreasing RAB3IP. CONCLUSION: LINC00943 inhibition alleviated MPP(+)-induced neuronal injury via miR-15b-5p/RAB3IP axis, indicating a potential target for treatment of PD.