Ovarian Cancer

XIST[LncRNA]

miR-106a[miRNA]

NA[mRNA]

Ovarian cancer tissues and cells

Homo sapiens (human)

Hum Cell. 2021 Mar;34(2):579-587. doi: 10.1007/s13577-020-00469-w. Epub 2021 Jan 5.

Upregulation of long noncoding RNA XIST has anticancer effects on ovarian cancer through sponging miR-106a.

CCK-8 assay;qPCR;RT-qPCR;RNA pull-down assay;Flow Cytometry assay;RNA pull-down;

Ovarian cancer (OC) is a highly malignant tumor. X inactive specific transcript (XIST) was identified as a cancer-related gene, while its therapeutic effect in OC was poorly defined. The present study was designed to investigate the effectual corollary of the lncRNA XIST in OC. RT-qPCR was used to detect the XIST and miR-106a expression levels of OC tissues and cell lines. OC cell apoptosis and proliferation were detected by flow cytometry, colony formation, and CCK-8 assays. Moreover, bioinformatics analysis was used to predict the targeted miRNA of XIST. The dual-luciferase reporter and RNA pull-down assays were then used to verify the interaction between miR-106a and XIST. OC xenograft nude mice were raised to measure tumor growth. Notably, OC tissues and cells exhibited low XIST levels and high miR-106a levels. The XIST upregulation decreased the OVCAR3 and CAOV3 cell proliferation and inversely promoted cell apoptosis. miR-106a targeted the XIST. Also, the miR-106a overexpression reversed the inhibitory effects of XIST on OC cell proliferation and apoptosis. Our in vivo results suggested that XIST was involved in tumor growth deceleration, while the miR-106a reversed the effect. To conclusion, the present study demonstrated that XIST suppressed OC development via sponging miR-106a both in vitro and in vivo.