Esophageal Cancer

AKT3[Circular RNA]

miR-17-5p[miRNA]

RHOC[mRNA]

esophageal cancer cells

Mus musculus (mouse)

World J Gastroenterol. 2021 Jan 21;27(3):240-254. doi: 10.3748/wjg.v27.i3.240.

Circular RNA AKT3 governs malignant behaviors of esophageal cancer cells by sponging miR-17-5p.

Luciferase reporter assay;

BACKGROUND: Recent studies have demonstrated that circular RNA AKT3 (circAKT3) plays a crucial role in regulating the malignant phenotypes of tumor cells. However, the potential effects of circAKT3 on esophageal cancer have not been investigated. AIM: To illuminate the role of circAKT3 in malignant behaviors of esophageal cancer cells and its underlying mechanism. METHODS: Clinical samples were collected to detect the expression of circAKT3. The role of circAKT3 in proliferation, migration, invasion, and apoptosis of esophageal cancer cells was evaluated using Cell Counting Kit-8, wound healing assays, Transwell assays, and fluorescence analysis, respectively. The target of circAKT3 was screened and identified using an online database and luciferase reporter assay. A xenograft nude mouse model was established to investigate the role of circAKT3 in vivo. RESULTS: In vitro assays showed that proliferative, migratory, and invasive capacities of esophageal cancer cells were significantly enhanced by circAKT3 overexpression. Furthermore, miR-17-5p was screened as the target of circAKT3, and miR-17-5p antagonized the effects of circAKT3 on esophageal cancer cells. Moreover, we identified RHOC and STAT3 as the direct target molecules of miR-17-5p, and circAKT3 facilitated expression of RHOC and STAT3 by inhibiting miR-17-5p. In vivo assays showed circAKT3 knockdown inhibited growth of esophageal cancer. CONCLUSION: CircAKT3 contributed to the malignant behaviors of esophageal cancer in vitro and in vivo by sponging miR-17-5p thus providing a potential target for treatment of esophageal cancer.